Category Archives: Digital Histology

Deep Learning, Digital Histology

Real time Data Augmentation using Nvidia Digits + Python Layer

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One of the common ways of increasing the size of a training set is to augment the original data with a set of modified patches. These modifications often include (a) rotations, (b) mirroring, (c) lighting adjustment, (d) affine transformations (sheering, etc), (e) magnification modification, (f) addition of noise, etc. This blog post discusses how to do the most trivial modification, rotation, in real-time using a python layer through Nvidia Digits. Given this code, it should be easy to add on other desired augmentations.

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Deep Learning, Digital Histology

Revised Deep Learning approach using Matlab + Caffe + Python

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Our publication “Deep learning for digital pathology image analysis: A comprehensive tutorial with selected use cases” , showed how to use deep learning to address many common digital pathology tasks. Since then, many improvements have been made both in the field and in my implementation of them. In this blog post, I re-address the nuclei segmentation use case using the latest and greatest approaches.

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Digital Histology

Dividing and re-merging large images (Humpty Dumpty)

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One of the challenges in working in digital pathology is that the associated images can be excessively large, too large to load fully into memory, as well as too large to use in common pipelines. For example, a Aperio SVS file that we’ll look at today is 60,000 x 42,600 pixels. If we tried to load such an image, in RGB space, uncompressed it would require ~7GB, making it too large to consider using in our deep learning pipelines as there wouldn’t be enough RAM on the GPU for both the data and the filter activations.

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Deep Learning, Digital Histology

Tutorial: A resolution adaptive deep hierarchical (RADHicaL) learning scheme applied to nuclear segmentation of digital pathology images

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This tutorial provides a tutorial on using the code and data for our paper “A resolution adaptive deep hierarchical (RADHicaL) learning scheme applied to nuclear segmentation of digital pathology images” by Andrew Janowczyk, Scott Doyle, Hannah Gilmore, and Anant Madabhushi.

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Deep Learning, Digital Histology

Use Case 6: Invasive Ductal Carcinoma (IDC) Segmentation

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This blog posts explains how to train a deep learning Invasive Ductal Carcinoma (IDC) classifier in accordance with our paper “Deep learning for digital pathology image analysis: A comprehensive tutorial with selected use cases”.

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Deep Learning, Digital Histology

Use Case 4: Lymphocyte Detection

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Typically, you’ll want to use a validation set to determine an optimal threshold as it is often not .5 (which is equivalent to argmax). Subsequently, use this threshold on the the “_prob” image to generate a binary image.This blog posts explains how to train a deep learning lymphocyte detector in accordance with our paper “Deep learning for digital pathology image analysis: A comprehensive tutorial with selected use cases”.

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